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Second, clinical trial data on nano-curcumin formulations remain sparse, and much of the evidence presented is derived from preclinical animal models or in vitro systems, which limits extrapolation to human applications

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siRNA transfection For Hk2 knockdown, the following siRNA sequences were employed: si Hk2 #1 (5′-GCGACATTTATAGGTGGAGAA-3′), si Hk2 #2 (5′-CATCACCCTGCTGGTTCTAAA-3′)